Agriculture and horticulture
A. Rafati; N. Valizadeh; F. Sefidkon; Y. Imani; F. Noormand Moaied
Abstract
Nepeta crassifolia Boiss. & Buhse, from Lamiaceae family, is an aromatic species with medicinal properties which is endemic to Iran. The seeds and aerial parts of this plant were collected from six different regions of East Azerbaijan province. After drying, the plant aerial parts essential oil was ...
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Nepeta crassifolia Boiss. & Buhse, from Lamiaceae family, is an aromatic species with medicinal properties which is endemic to Iran. The seeds and aerial parts of this plant were collected from six different regions of East Azerbaijan province. After drying, the plant aerial parts essential oil was obtained by hydrodistillation and their compounds were identified by GC and GC/MS. The collected seeds, after producing greenhouse seedlings, were planted in the field in a randomized complete block design with three replications. In the second year after planting, the flowering shoots were harvested, dried, and distilled. The results showed that there was significant differences among essential oil percentage of natural habitat samples which the highest amount belonged to "Marand" region, but the farm essential oil samples did not differ with each other significantly. The compounds nepetalactone I (4aα, 7α, 7aα) and II (4aα, 7α, 7aβ), globulol, germacrene B, caryophyllene oxide, and 1,8-cineole were identified as the main essential oil compounds of this species. The highest amount of nepetalactone I or II under the natural habitat (69% and 21.7%, respectively) and field (27.3% and 51.5%, respectively) conditions belonged to "Misho" region in "Marand". "Sarab" region also had the highest amount of nepetalactone II under the natural habitat and farm conditions compared to the other samples. The highest amount of 1,8-cineole (21.8%) in the oil was also obtained from the natural habitat of "Kharil" region.
Y. Imani; R. Razban Haghighi; F. Sefidkon; M. Naderi
Abstract
The current study was aimed to investigate the essential oil of Thymus pubescens Boiss.et Kotschy ex Celak chemotypes in five regions of E-Azerbayjanprovince. Samples were collected at the flowering atage. Hydrodistillation method was used to obtain essential oil. Quantitative and qualitative analysis ...
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The current study was aimed to investigate the essential oil of Thymus pubescens Boiss.et Kotschy ex Celak chemotypes in five regions of E-Azerbayjanprovince. Samples were collected at the flowering atage. Hydrodistillation method was used to obtain essential oil. Quantitative and qualitative analysis was done by GC and GC/MS methods. Twenty six compounds were determined in the essential oil of experimental sites. Cluster analysis, based on these components with WARD method, showed that five areas were divided into three clusters, so that the first cluster was related to the Sahand area chemotype, the second cluster was related to the Mishoo chemotype and the third cluster included common chemotype in Malekan (Egdelou), Kaleibar (Yuzband) and Maraghe (Kordedeh). Our results showed that the highest and lowest amount of essential oil was recorded for the first chemotype (Sahand, 0.31%) and the third chemotype (Egdelou, 1.34%), respectively. According to the Principal Coordinate Analysis the major compounds of essential oils were as follows: in the first chemotype: р-cymene (4.3%), n-nonanal (9.8%), methyl ether thymol (10.6 %), thymol (30.9 %) , thymyl acetate (13.5%); in the second chemotype: p-cymene (4.1%), γ-terpinene (6.2%), carvacrol (82.1%); in thr third chemotype p-cymene (7.2-1.6%), γ-terpinene (6.5-2.2%), thymol (65.6-27.6%), carvacrol (25.4-3.7%).
Y. Imani
Abstract
In order to investigate the quantitative changes of essential oil during growth period (vegetative, flowering, after flowering) in Melissa officinalis, samples were collected from Arasbaran and Malekan for two years (2001 and 2002). All samples were dried in laboratory condition and then essential oils ...
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In order to investigate the quantitative changes of essential oil during growth period (vegetative, flowering, after flowering) in Melissa officinalis, samples were collected from Arasbaran and Malekan for two years (2001 and 2002). All samples were dried in laboratory condition and then essential oils were extracted by steam distillation method. The oils were dried over anhydrous sodium sulfate and weighted. Combined analysis of data was done with C.R.D design in 3 replications. Combined analysis of data for Melissa officinalis indicated that effects of year, location and phenology phases weren’t significantly different, but the interaction of (year × location) and (location × year × phenology phases) were significantly different at 1% probability level. Means were grouped using L.S.D (5%). Based on the results of this study, essential oil yield in Malekan was higher at first year at flowering stage (0.197%). Mean of interaction (year × location) showed that oil yield of Melissa officinalis in Malekan at first and second year were respectively (0.108 and 0.080%), and in Arasbaran were (0.035 and 0.018%). The results of the two years showed that the average oil content at first year was more than that at second year.
